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SCARLESS-WIZARD

Scarless DNA assembly wizard

SCARLESS-WIZARD (Scarless DNA assembly wizard) is a API endpoint to automatically compute synthesis fragments compliant with Gibson, In-fusion, Surevector or NEB assembly protocols. Would you like to try it?

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Parameters

is_circular: Indicates the topology of the input DNA. Please set to true if the target sequence is circular, else false

scarless_protocol: Currently supported protocols: Gibson, In-fusion, Surevector or NEB assembly protocols

min_overlap_length: Minimum length of the overlap regions between two adjacent fragments

max_overlap_length: maximum length of the overlap regions between two adjacent fragments

min_fragment_length: Minimum length of the synthesis fragment computed

max_ fragment_length: Maximum length of the synthesis fragment computed

target_fragment_number: The ideal number of fragments computed. By default, the algorithm minimizes the number of fragments.

amplification_primers: indicates whether amplification primers shall be computed for each synthesis fragment. Please set to true if you compute amplification primers, else false

min_primer_length: Minimum length of the amplification primers. This parameter is valid only if amplification_primers is true

max_primer_length: maximum length of the amplification primers. This parameter is valid only if amplification_primers is true

min_primer_Tm: Minimum primer Tm. This parameter is valid only if amplification_primers is true

max_primer_Tm: Maximum primer Tm. This parameter is valid only if amplification_primers is true

Example

Input payload

{
    "is_circular": True,
    "scarless_protocol": "Gibson",
    "min_overlap_length": 30,
    "max_overlap_length": 40,
    "min_fragment_length": 500,
    "max_ fragment_length": 1000,
    "target_fragment_number": 5, 
    "amplification_primers": True,
    "min_primer_length": 15,
    "max_primer_length": 40, 
    "min_primer_Tm": 55,
    "max_primer_Tm": 65
}